FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180372
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12950.043.3710671
TCTGTCG14600.039.9447488
TCGCTCT14450.035.875024
TCTCGCT16700.031.4761562
GTATTAT11250.031.3630921
CGCTCTG15350.030.9573025
CTCGCTC16400.029.8533153
CTGTCGC18750.029.1836649
GTCTTAC13950.027.3575341
GTATTAG10900.027.0852381
GTCTTGC27750.026.4674741
GTGTAAA20350.026.18441
GTGTTAG23000.025.6720961
GTATTGA11650.024.1053811
GTCTCAC35600.023.0584551
TAACACG6650.022.7365824
GTATAAC6700.022.5693871
GTATAAG10650.022.312061
GTATTAC7450.022.2303811
GGAGTGC68750.021.6782955
Produced by FastQC (version 0.11.5)