FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3708743
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17200.033.4878658
GTCTCGC14700.033.337081
CTGTCGC20000.029.5195529
GTCTTGC35650.027.8968721
GTATTAG14600.027.1484971
TAATACT13600.025.9407834
TCGCTCT17450.025.5812744
GTGTAAA28450.025.3310781
GTCTTAC17500.024.708661
GTGTTAC16300.023.8749311
GTATTAA12400.023.8285791
GTATAGA13350.022.6727391
GTTTCAC61000.022.4258923
TCTCGCT20300.022.3616732
CTCGCTC20350.021.9357853
GTGTAAC11850.021.893751
TTACACT21250.021.006744
TTCACCG12750.020.8937995
GTATTAC11050.020.8700581
CGCTCTG20950.020.6202135
Produced by FastQC (version 0.11.5)