FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868248
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.039.5996748
GTCTCGC12650.035.865541
CTGTCGC14400.033.4997259
TCGCTCT14250.032.3365754
GTATTAA10250.031.616661
GTCTTGC26450.030.4943731
CCTATCG1902.2090353E-730.3155389
GTATTAG9450.029.7207761
GTCTTAC12700.027.2185421
CGCTCTG16450.027.1365535
TCTCGAT10100.027.0888867
TCTCGCT16050.026.9217662
CTCGCTC15850.026.3467863
CTCGATC11050.026.0631338
GTGTAGA15950.024.3814981
TCGATCT11600.024.2066979
GTACTAC5100.024.0052431
GTGTAAT13250.023.9146561
GTATAGT8400.023.1479131
GTATTAC8550.022.7418081
Produced by FastQC (version 0.11.5)