FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868248
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13900.036.7763258
GTCTTGC27300.032.971621
GTCTCGC12450.030.0764731
GTATAAC6650.029.237151
CTGTCGC18050.027.9219429
GGTATAC8200.026.3451291
GTCTTAC11600.025.4518151
TCGCTCT14450.025.4113124
GTACTAA6600.025.0943981
GTATTAG9850.024.8562491
TTGCTCT36850.024.8136714
GTATTAA10450.024.8072781
GTGTTAG19800.024.7307111
GTGTTAC12650.023.9084631
CTTGCTC36400.023.5380423
GTATTAC9300.023.2290381
CGCTCTG15450.022.8345435
TCTCGCT15500.022.7644582
CTCGCTC16550.022.6219543
AGTACCG1600.00226566422.49965
Produced by FastQC (version 0.11.5)