FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319604
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15350.041.2761768
TCGCTCT15150.039.446074
GTCTCGC15150.037.1078071
CTCGCTC16400.032.927273
CTGTCGC19750.031.7159219
TCTCGCT17600.031.5227342
CGCTCTG17850.031.058775
GTCTTGC31050.030.6404881
GTATTAA12200.025.9941831
GTTAGCC17850.023.3952673
GTGTTAG26500.023.1183241
GTCTCAC41800.023.1053121
GTACCGG2502.4015408E-623.063931
GTATTAG13200.022.9328841
GTGTTAC16050.022.4532031
GGTATAC7750.022.3199331
GTTACCC11600.021.7244263
GAGTGCA79850.021.2798636
CTTGTCG3758.427378E-921.1196429
GTATATA15500.020.9249361
Produced by FastQC (version 0.11.5)