FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793210
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14450.042.8504458
GTCTTGC36350.034.6742631
GTCTCGC16350.034.3597871
CTGTCGC19850.030.4679689
TCGCTCT18250.029.9830254
TCTCGCT20300.025.8996982
CTCGCTC20400.025.772743
CGCTCTG20750.025.3296685
GTATAGA13150.025.1944961
GTATTAT13150.024.6467911
TTGCTCT50650.024.1654134
GGTATAC8050.024.1569161
GTGTAAC13600.023.8312721
CTTGCTC48200.023.7584443
GTCTCAC46200.023.5401151
TAATACT12800.022.4995984
GTATTAG15400.021.9811671
GTGTTAC17850.021.788591
TTATACT11900.021.7811224
GGATAAC12100.021.428451
Produced by FastQC (version 0.11.5)