FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793210
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15900.039.3956348
GTCTCGC15900.035.813171
CTGTCGC20550.031.8827299
TCGCTCT17400.031.8615884
GTCTTGC34900.029.3275451
CGCTCTG19800.025.8177935
TCTCGCT21400.025.5896072
GTCTCAC45350.024.7947711
CTCGCTC21800.024.770273
GTGTTAC15400.023.870541
TTACACT19300.023.1291874
GTACTAG8450.023.0313741
GTATAAG10400.022.8714371
GTATAAC7700.022.4663931
GTATTAC9950.022.4569841
GTGTAAA27250.022.2190551
GTATAGG10650.021.6577421
GTCTTAC16650.021.6455481
GGGTTTC65500.021.5688781
GTATATA18050.021.5640011
Produced by FastQC (version 0.11.5)