FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682370
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15100.046.251058
CTGTCGC19500.036.9225929
GTCTCGC15300.036.715371
TCGCTCT16550.033.9330864
GTCTTGC36000.033.008531
TCTCGCT18200.030.8651182
CTCGCTC18700.028.8844683
GTGTTAC15800.028.716281
GTCTTAC16750.027.947521
GGTATAC8550.027.7966561
GTGTAAC14750.027.3426571
CGCTCTG19900.027.135325
TTCACCG10700.024.8968665
ACCGTGT11750.024.5103158
GGGTTTC62550.024.2940481
GTTTCAC57100.024.2164153
GTCTCAC42450.023.2427541
GTTACAC10400.022.8520573
TAGGGCG2852.97543E-722.7365425
GTATTAT15000.022.565831
Produced by FastQC (version 0.11.5)