FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311618
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.034.8693738
GTCTCGC13850.033.800251
GTCTTGC30050.033.7933351
TCGCTCT15850.030.434824
CTGTCGC14300.029.7057959
GTATAAG10900.025.7688181
TCTCGCT17400.025.661332
CGCTCTG17900.024.9381315
GTGTTAC13300.024.909361
TTACGCT2401.6995054E-623.9995964
TAATACT10850.023.8894
CTCGCTC17900.023.737543
GTGTAAC11100.023.3580131
GTATTAC8100.023.1177021
GTGTATA16650.022.9254591
GGTATAC6750.022.4063911
GTCTCAC40550.022.0235441
GTATAAT10400.021.467661
CTGTTGC33850.021.2699539
GTGTAAG19050.021.1713921
Produced by FastQC (version 0.11.5)