FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311618
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.043.1434758
GTCTCGC14700.037.2578161
TCGCTCT17400.033.103074
CTGTCGC18950.031.1553159
GTCTTGC30150.030.8335531
GGTATAC6800.029.67361
CTCGCTC19900.027.858983
TCTCGCT19950.027.4485652
CGCTCTG18250.027.2216075
GTGTTAG22750.025.0245821
GTACTAT7950.024.4747241
GTATTAA10000.023.7812711
GTATTGC10250.022.4981731
GGGTTTC55450.022.4835931
TTCACCG10550.021.8386145
GTCTAGA17150.021.0100461
TTGCTCT42350.020.9112134
GTCTCAC37950.020.6983571
GTTTCAC51850.020.6902163
GCGTTAC2455.361661E-520.5898481
Produced by FastQC (version 0.11.5)