FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618608
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15250.042.9627728
GTCTCGC15050.038.2872621
TCGCTCT17400.035.1714634
CTGTCGC19450.034.7959759
GTCTTGC32300.033.4494931
CTCGCTC18950.030.7872283
TCTCGCT18750.030.7314832
CGCTCTG19450.028.5030865
TTCACCG10700.026.915165
ACCGTGT11750.025.7354748
GTATAGG10300.025.1748031
GTGTAAC11650.024.7306141
GTGTAAA22600.023.9030671
TTGCTCT47050.023.565784
CCGTGTT13300.022.7362259
GGGTTTC63000.022.0656931
GTACTAC6700.021.5008681
GTTTCAC55450.021.4327163
GTCTAGA17250.021.2952081
GTGTATA20000.021.2482341
Produced by FastQC (version 0.11.5)