FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618608
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14750.037.625891
TCGCTCT18200.030.0654684
TCTGTCG15850.029.0721028
GTCTTGC33000.027.0829411
CTGTCGC19200.026.6245849
CGCTCTG19750.026.6122445
TCTCGCT20500.026.0128442
CTCGCTC20300.024.4725273
GGTATAC7850.023.8721621
GTCTTAC15100.022.9114381
GTCTCAC39850.022.246661
TTACACT19650.021.9843924
GTACTAG8000.021.6226711
GTGTAAG18300.021.26821
GTGTTAG27800.021.2596991
TTGCTCT47650.021.153924
GAGTGCA84700.021.0811376
GTGTTAC14750.021.011861
GTACTAT8300.020.8411271
TCTGTCA47250.020.5711088
Produced by FastQC (version 0.11.5)