FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3807357
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14450.039.3623288
GTCTCGC16700.035.3681871
TCGCTCT17600.031.9082814
GTCTTGC35100.030.3717631
CTGTCGC20200.028.8705569
CTCGCTC19500.028.4420013
TCTCGCT20700.027.4891172
CGCTCTG20600.026.5624335
GTGTAAC12500.025.354681
GTATAGC9600.024.0101131
GTCTTAC16000.023.4098591
GTATTAT15650.023.0128881
GTGTAAA23200.022.0437681
GTGTTAG31450.021.9870031
GTACCGT1650.002702241321.8273741
GTACTAC7000.021.60911
GTATAAT12700.021.5523851
GTATAGG10850.021.2439691
TACACCG2755.540396E-620.9449235
GTATTAA12600.020.5800951
Produced by FastQC (version 0.11.5)