FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3807357
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16950.042.0951271
TCGCTCT16850.039.738374
TCTGTCG15300.037.1759958
TCTCGCT21150.033.7296032
CTCGCTC20700.033.3908773
CGCTCTG19650.032.2438625
GTCTTGC36350.031.3270171
GTATTAA12450.028.9445631
CTGTCGC19750.028.435089
GTCTTAC15650.027.6314221
GTGTTAC16550.024.8223671
GCGTTAC2101.6295853E-524.0239871
TTGCTCT47950.022.9736394
GTATAGC7750.022.319061
GTACTAT8750.022.2393471
GTCTCAC44200.021.6868131
TAATACT12300.021.658264
CTGTTGC41300.021.4428279
TCTGTCA50550.021.3647128
CTTGCTC48100.021.105763
Produced by FastQC (version 0.11.5)