FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565875
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14600.035.5445941
GTCTCGC14400.035.537741
GTCTTGC35450.032.124381
TCGCTCT16050.031.8499014
TCTGTCG15250.031.1605368
CTGTCGC17400.027.3102429
TCTCGCT19600.026.102032
GTATTAA11950.025.935491
GTATTCG2001.1160328E-525.2267881
GTGTAAG17100.024.8685131
CTCGCTC20300.024.1177963
GTGTAAC12000.024.0255131
GTGTAAT16450.023.6603851
GTATTAC9650.023.1541211
TTGCTCT47450.023.0638664
GGTATAC7700.022.4654141
GTCTAGC17750.022.3335741
GTGCTAC12700.022.133741
GTCTTAC16350.022.0417561
CTTGCTC48150.021.9809173
Produced by FastQC (version 0.11.5)