FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071445
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12050.041.2256288
GTCTCGC14300.037.01811
TCGCTCT14450.036.3726044
GTCTTGC24500.031.0778181
GTATTAC6350.030.833111
CTGTCGC18650.030.1107129
TCTCGCT17700.029.7312892
GGTATAC6500.029.005961
CGCTCTG16450.028.4489885
CTCGCTC17550.027.8965933
GGGTTTC44500.027.0505051
GTGTTAG20250.026.4992731
GTATAAC5550.026.1314981
TTGCTCT32500.025.2546224
GTGTTAC11400.024.8077321
GTATTGC6750.024.7087821
TAATACT7000.024.684974
TTCGCAC1751.3172672E-424.684973
GTCTCAC31200.024.4040551
GTTTCAC40100.024.238673
Produced by FastQC (version 0.11.5)