FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974091
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13750.045.554538
GTCTCGC17150.042.477591
TCGCTCT18050.039.8926164
TCTCGCT19500.034.7333952
GTCTTGC31850.034.6485141
CTCGCTC20000.032.7821923
CTGTCGC20200.031.0086549
CGCTCTG22200.030.160955
GTATTAG12550.027.5867251
TTGCTCT42100.025.8264394
GTGTTAG23900.025.0485631
GTGTAAC9900.024.7711621
GGTATAC8400.024.0425971
CTTGCTC42100.023.7880763
TCTCGAT13650.023.735317
GTATTAA10500.023.3556671
TTATACT10200.023.2961144
GTCTTAC13050.023.2135411
GTCTCAC40750.023.1870921
CTCGATC13850.022.8727268
Produced by FastQC (version 0.11.5)