FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974091
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17350.041.9078258
GTCTCGC16800.037.9478651
TCGCTCT18550.035.317274
GTCTTGC32700.033.2320751
CTGTCGC25550.030.993819
TATACCG1255.3739804E-428.7960725
GTATAAC7300.028.7798881
CGCTCTG22100.028.3400215
CTCGCTC21850.028.3358763
TCTCGCT23000.028.1714882
GTACGAC1358.122281E-426.8318211
GTGTTAG24150.026.0985321
GTTAGCC19950.025.621533
GTGCTAC11600.024.9813521
CTATACT9600.024.7475574
GTCTTAC13300.024.511781
GTACCGT2951.5421392E-824.4034486
TTATACT10900.023.7774684
GTGTTAC13750.023.7095761
TTGCTCT45400.023.4690484
Produced by FastQC (version 0.11.5)