FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305428
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG20700.042.783478
GTCTCGC21750.036.484681
GTCTTGC43600.035.4082451
TCGCTCT22550.032.2568934
CTGTCGC28200.032.1696479
GTCTTAC17050.028.771441
TCTCGCT26100.028.7162172
GTGTTAG32700.028.4589631
GTATTAC10200.028.290261
TTGCTCT57600.027.6323284
GTATTAA12550.027.0166321
GTGTTAC19750.026.6644651
CTCGCTC27450.026.2523063
GTTTCAC68450.026.2141443
GGGTTTC75350.025.7540861
GTATTAG17400.025.7051141
GTACTAC9550.025.6834071
CGCTCTG27250.025.6298735
GTTAGCC27100.025.261793
TACACTG28500.024.0004845
Produced by FastQC (version 0.11.5)