FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305428
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG21650.045.22348
GTCTCGC21200.043.736651
GTCTTGC43050.034.494681
TCGCTCT23950.033.967164
CTGTCGC32550.032.954719
TCTCGCT28300.029.2551142
CGCTCTG27350.029.2178215
GTATTAC11650.027.9806611
GGGTTTC67750.027.9063221
CTCGCTC26900.027.833473
GTTTCAC62950.026.5325223
CCGTGTT15250.026.4362819
GTGTTAC19050.025.0969141
GTCTCAC52000.025.0749781
GTCTTAC17400.024.9789071
TAATACT11250.024.3174114
TTCACCG13750.024.0844825
TACACTG28400.024.0817185
GGTATAC10250.024.0284881
GTGTTAG29650.023.6983661
Produced by FastQC (version 0.11.5)