FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5426143
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG20000.043.919918
GTCTCGC21950.043.7072941
TCGCTCT24100.038.2508054
CTGTCGC27450.033.5730829
TCTCGCT26400.033.3011972
GTCTTGC46500.032.266151
CTCGCTC29050.029.7653543
GTATTAG15900.029.4885221
GTCTTAC19450.028.9275591
CGCTCTG30250.027.6098615
GGGTTTC74900.025.6174931
GTTTCAC66500.025.3553813
GTATTAT15650.024.8895031
GGTATAC11350.024.7859481
TAATACT13050.023.7304334
TTACTCT27250.023.5218354
GTGTTAG36500.023.1222341
TTGCTCT61650.023.0134034
TACACTG30350.023.0114865
GTGTTAC22300.022.9662591
Produced by FastQC (version 0.11.5)