FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4286398
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15550.048.1562278
GTCTCGC18350.039.7097321
CTGTCGC21950.037.394679
GTCTTGC32950.033.9381331
TCGCTCT18900.033.915594
TCTCGCT21600.030.3639222
TAATACT10000.030.2496514
CGCTCTG20700.028.1750135
CTCGCTC23550.028.153783
GTCTCAC40900.027.3413581
TTCACCG10550.026.6171555
GGGTTTC62600.026.3920331
GTGTTAG27250.025.9460531
TTACACT16100.025.498824
GTTTCAC56950.025.182523
GAGTTAC11800.025.0676441
GTACTAC6050.025.042381
GTATTAC9000.024.8502561
GTATTAG13850.024.4827251
TTGCTCT45550.024.3502524
Produced by FastQC (version 0.11.5)