FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3469818
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13950.040.3519631
TCGCTCT13550.038.789924
TCTGTCG11400.038.5298968
TCTCGCT16750.032.6971442
GTCTTGC24050.032.4080541
CTCGCTC16750.031.8357753
CGCTCTG16950.031.436555
CTGTCGC17400.028.5514989
GTCTAAG9500.025.0688631
GTCTCAC31400.024.8220941
GTATTAT9650.024.6791921
TTACACT13350.024.2698214
GTGTTAC11600.024.2633551
GTATTAA8150.023.9083961
CAATACT9050.023.8675584
TTGCTCT34300.023.5103574
GTATTGT11900.023.0452231
GTACCGA1650.002672422221.8690721
TCTGTCA33600.021.4305658
GTGTAAA16850.020.5582261
Produced by FastQC (version 0.11.5)