FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4427092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17300.043.2881368
GTCTTGC34850.037.068871
GTCTCGC18550.035.7934041
TCGCTCT20400.032.8231624
CTGTCGC23400.032.000739
TCTCGCT21700.028.8908422
CTCGCTC23400.027.714543
CGCTCTG24100.027.1897985
GTATAAT10650.026.4285961
GTCTCAC41900.026.3533881
GTGTAAA21950.025.6459711
TTGCTCT44750.025.4209464
TTATACT11350.025.3741664
GTCTTAC15550.025.062391
GGTATAC7950.024.51071
GTGTTAG27200.023.8799081
GTGTAAC10600.023.1489931
TCGATCT18750.022.6565159
GTTTCAC56250.022.4179823
CTTGCTC47300.022.3942853
Produced by FastQC (version 0.11.5)