FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4427092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17750.036.9102678
GTCTCGC19950.034.903351
GTCTTGC34050.033.444241
TCGCTCT20950.031.616794
GTCTTAC12700.029.6987671
CGCTCTG22450.028.8629115
CTGTCGC25650.028.6297119
TCTCGCT23600.028.4047152
GTATTAA10150.027.1554091
CTCGCTC24550.026.3939883
GTATTAG11000.026.3758281
GTGTTAC14750.025.5711421
GTATAGT11100.025.4847531
TTGCTCT44450.025.1057914
GTACTAC7450.024.340111
TACACCG3902.1827873E-1123.9989785
GTATTGA12000.023.5733971
GTGTAAT16350.023.5123981
TTACACT17800.023.4596734
GTATAAT12150.023.2823661
Produced by FastQC (version 0.11.5)