FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502688
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.044.0479778
GTCTTGC19900.035.884371
TCGCTCT9950.035.4580234
GTCTCGC11500.033.8703081
GTATTAC5400.032.058311
CTGTCGC12750.031.624199
TTGCTCT26400.028.9096934
GGGTTTC32500.028.630541
GTATTAG7650.028.2867451
CTCGCTC11400.027.8100553
TAATACT6150.026.9273914
ACCGTGT6750.026.6672218
TCTCGCT11950.026.5300942
GTTTCAC31300.026.4732693
GTATTAA5850.025.8932531
GGTATAC5300.025.8583561
CTTGCTC26850.025.7620833
GTGTTAC8450.024.7550871
CTTTACG1751.3167161E-424.6862289
CGCTCTG13150.024.6392965
Produced by FastQC (version 0.11.5)