FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502688
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9950.037.1654321
GTCTTGC19650.033.210241
TCGCTCT11300.032.492544
GTGTAAC5950.031.6846181
TCTGTCG10350.029.910258
TACACTG11650.029.0444985
CTCGCTC13300.028.689053
GTCTCAC23850.027.9699361
TAATACT6200.027.8683644
TCTCGCT14200.027.4040072
CGCTCTG13600.026.4681175
GTGTTAG12700.026.2631111
GTATTAG6400.026.057931
GGGTTTC34900.025.9702821
CTACACT10300.025.8616624
GTGTAAT9000.025.7809871
GGTATAC4550.025.4976791
TCTGTTG21300.025.3497458
TTGCTCT27200.025.144714
GTTTCAC33050.025.0506083
Produced by FastQC (version 0.11.5)