FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6190552
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG23950.044.7966278
GTCTCGC25550.035.023071
CTGTCGC33550.034.3362929
TCGCTCT27300.032.7028054
GTCTTGC53050.031.0150781
TCTCGCT29100.029.4671382
TTACACT26800.028.7458364
CTCGCTC31300.027.6246173
GTATTAA14150.026.5198041
GTATAAG16700.026.359481
GTTTCAC82800.025.8455333
CGCTCTG34300.024.9814385
TAGTACT11600.024.8272134
GGGTTTC94600.024.6396661
TACACTG34950.024.516835
TTGCTCT68500.024.4901454
GTGTTAG37850.024.4043661
GTCTTAC24250.024.4020061
GTTAGCC32150.023.9807173
TAATACT13700.023.6492794
Produced by FastQC (version 0.11.5)