FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5881269
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG22500.038.4002348
GTCTCGC22200.035.760121
TCGCTCT23900.031.9308074
GTCTTGC50800.030.5445691
CTGTCGC30700.029.0801269
GTTTCAC75750.027.4895763
GGGTTTC82100.026.020031
TTCACCG17800.025.8877985
TTGCTCT62000.025.7788314
ACCGTGT17050.025.759698
GTGTTAG37100.025.4833561
TCTCGCT27500.025.4161832
TAATACT14800.025.2955174
GTATTAG18000.025.2596471
GTGTTAC20200.025.0095521
GTATTAA14450.024.9724641
GTATTAT14800.024.8695371
CGCTCTG27650.024.7379465
GTCTTAC19850.024.723371
GTATAAC9450.024.4386621
Produced by FastQC (version 0.11.5)