FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5088345
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC18250.039.7391661
GTCTTGC39700.036.9013671
TCTGTCG18550.036.4827358
TCGCTCT22250.033.005834
TCTCGCT24400.028.6548232
TAATACT13500.027.199254
CTGTCGC27800.026.9334499
CGCTCTG24900.026.8908235
GTACTAG9300.026.5141411
CTCGCTC24850.026.3654693
GGGTTTC71300.025.6325951
GTGTTAG30600.025.5966971
GTTTCAC63500.025.2843423
TTGCTCT53450.024.6503944
GTCTTAC16500.024.6141991
GTATTAC10350.024.5250171
GTATTAA12550.024.2709731
GTCTCAC48800.023.7783531
ACCGTGT16200.023.5539788
TTACACT21150.022.4674634
Produced by FastQC (version 0.11.5)