FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5224905
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG22450.044.2584348
GTCTCGC22450.043.0836221
TCGCTCT24350.038.438764
CTGTCGC30750.035.5879259
TCTCGCT26600.033.321682
GTCTTGC42700.032.7942431
CGCTCTG27900.031.2258645
CTCGCTC28550.029.5310573
TTGCTCT56450.028.6974664
GTCTTAC18600.026.7768751
GTGTTAC20300.025.2456191
CTTGCTC56900.024.4424253
GTGTTAG34050.024.3783571
CTCGATC21100.024.2275338
GTCTCAC55950.023.9958741
CTGTTGC47550.023.6199139
TCTGTCA57150.023.55918
GTTTCAC67450.023.3970683
TTACACT20100.023.283184
TCTCGAT21800.023.119317
Produced by FastQC (version 0.11.5)