FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626787
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13750.043.462748
GTCTTGC31000.034.4528351
GTCTCGC15300.033.0165371
TCGCTCT16050.031.8502664
CTGTCGC18800.031.4014599
TCTCGCT15500.030.6841032
TAATACT8250.028.799824
GTATAAT9350.028.5571611
GTATTAT10550.028.045061
TTGCTCT38200.027.706634
TCTCGAT12900.027.3494197
GTCTTAC11450.027.1011621
CTCGATC13000.026.585188
GTGTTAG21000.026.4603961
CTCGCTC16250.026.1619343
CTTGCTC39200.025.9181653
GTATTAG10700.025.6285921
TCGATCT13300.025.4413439
GTCTCAC37600.023.6070771
GTGTATA17000.023.347411
Produced by FastQC (version 0.11.5)