FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626787
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14300.040.0604171
TCTGTCG13050.038.0671468
TCGCTCT15750.034.7421574
GTCTTGC28150.030.1392141
TCTCGCT19150.028.6034132
CTCGCTC18900.028.1899073
GTGTTAC10600.028.0480351
CTGTCGC19200.027.7486749
CGCTCTG18100.027.446965
GTACTAC5200.026.4956571
GTATTAG9450.026.0898481
GTATAAT10650.025.8736931
TTGCTCT38250.025.0347884
GTGTAAA17900.024.711621
TACCCGT3251.6134436E-924.3687385
GGGTTTC48900.024.3197651
GTGTAAC8950.024.306511
TTACACT13900.023.8268594
GTGTTAG21000.023.1355531
GGTATAC6600.023.0727731
Produced by FastQC (version 0.11.5)