FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429196
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT9550.039.9567534
GTCTCGC10300.037.8163381
TCTGTCG9050.037.390998
GTATTAT6700.034.4507061
TCTCGCT10900.033.05022
GTCTTGC19150.031.2631151
CTGTCGC11800.031.1182219
CTCGCTC11350.029.1994533
GTATTAA4950.029.1439081
GTATTAG6950.029.060041
CGCTCTG12450.027.7580875
GTCTTAC7750.026.9910161
GGGTTTC34250.026.9570481
TAACACT8300.026.8906484
GGTTTAC5400.026.7152481
TAATAGT4100.026.3405634
ACCATGT24300.025.7768978
GTTTCAC30950.025.1406143
TTGCTCT25350.024.993234
TTATACT4350.024.8267384
Produced by FastQC (version 0.11.5)