FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429196
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.044.3646138
GTCTCGC10500.042.8119661
TCGCTCT11100.035.672574
GTCTTGC19900.032.7908131
CTCGCTC12950.030.0205463
CGCTCTG12750.029.92685
TCTCGCT13500.029.891752
GGTATAC4850.028.403681
GGGTTTC35600.028.3091981
CTGTCGC14250.028.292859
GTCTTAC7800.027.8862041
GTTTCAC30950.026.2852693
ATGGCGT9450.025.9025046
TTGCTCT25850.025.3440174
GTATAGG6350.025.1195431
GTATTAA6600.024.1680451
AACCGTG4201.8189894E-1223.99847
GTCTCAC23300.023.9605921
GTACTAC3352.1118467E-923.807331
GTATTGA6100.023.7718491
Produced by FastQC (version 0.11.5)