FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356715
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10000.039.8990251
TCTGTCG9400.039.0606888
GTCTTGC18550.036.3696061
TCGCTCT10600.035.997874
GGTATAC5250.033.1628231
CGCTCTG12500.030.5255455
TCTCGCT13550.029.7919882
TTGCTCT24350.029.2713664
GTATTAA5750.029.017471
CTCGCTC13300.028.1481113
GCGTAGT3102.7284841E-1128.0814231
GTATTAC3403.6379788E-1227.737291
GCGTACT1050.00792904427.4613252
GTATTAG5850.027.2813841
GTACTAA3950.025.7116811
GTCTTAC8200.025.6556911
GTCTCAC24050.025.6391371
GTGTTAA7700.025.4373931
GTGTTAG13450.025.3498361
GGGTTTC31300.025.2628151
Produced by FastQC (version 0.11.5)