FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810461
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10650.042.5912441
TCTGTCG10700.042.392228
TCGCTCT11950.039.7654084
GTCTTGC20600.033.9026681
GTCTTAC7700.032.7270361
CTCGCTC13600.031.7644793
CGCTCTG12900.031.2555895
TCTCGCT14350.030.1043152
GTATTAG8250.028.7997951
GTATTAT8000.027.8998011
GTATAAG9100.027.6921081
CTGTCGC17700.026.4409629
GTACTAT4650.026.3223921
GTGTAAA14750.025.8709981
ACCGTGT9000.024.7998228
AGTACCG1751.3170223E-424.685545
TTACTCT12350.024.4856554
GTATTAA7450.024.1609021
GTGTAAT9650.023.8754751
GTGTTAC9400.023.744511
Produced by FastQC (version 0.11.5)