FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810461
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.038.571838
GTCTCGC11150.034.889561
GTCTTGC21950.031.8356551
TCGCTCT13000.029.907474
GTGTTAG17550.027.5026321
CGCTCTG13600.026.9998025
GTACTAT5500.026.1965371
GTCTCAC28500.025.782911
TATACTG7600.025.5787585
GTTAGCC13900.025.3811073
TTGCTCT29250.025.3536594
GTGTTAT10850.024.56681
CTCGCTC14600.024.1642063
GTCTCGA22950.023.842966
GTATATA11850.023.7095241
CTCGATC11550.023.6885578
GTGTAAT10150.023.4220281
TGTTAGC15450.023.3007982
TCTCGCT15550.023.1509532
TATAATA7800.023.0767522
Produced by FastQC (version 0.11.5)