FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922982
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.045.0909588
GTCTTGC24950.034.691611
CTGTCGC13900.033.6690989
GTCTCGC13850.033.330661
TCGCTCT13000.032.123114
GTATTAA6200.029.084531
ACCGTGT9500.026.5263448
GTGTTAA9800.025.7605841
TCTCGCT14600.025.66232
TCGCACT3659.094947E-1225.6438644
GTTTCAC38250.025.2395323
TTGCTCT32000.024.9750294
GGGTTTC42000.024.9018971
CTCGCTC15400.024.7949393
GTATTAT9450.024.424851
TAATACT5950.024.2017064
TTCACCG8050.024.1490945
GTACTAT6650.023.8624361
TTACACT12150.023.703734
TTATACT6400.023.6250254
Produced by FastQC (version 0.11.5)