FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922982
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC22850.035.5627671
TCTGTCG11600.034.13488
GTCTCGC13550.033.733781
TACACCG2601.200533E-1030.4582235
TCGCTCT14050.030.2326224
TAATACT7300.029.5868344
GTATTAG7400.029.4139481
GTACTAC4500.029.021761
TATAGCG1000.00625348828.8299082
GTATAAG7700.027.3256841
GTGTTAG16300.026.7071421
TACACTG15500.026.4745395
TATACTG6800.026.4677095
CGCTCTG16950.026.3334185
GTGTAAC7200.026.20021
ACCGTGT8150.025.6172838
TCTCGCT16950.025.5131932
GTATTAA6900.025.2363131
GGGTTTC38700.024.9347171
CTCGCTC16750.024.9294833
Produced by FastQC (version 0.11.5)