FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326918
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9350.032.408411
TCTGTCG7150.030.2104438
GTGTTAG12500.030.0132751
TCGCTCT9700.029.6913624
GTCTTGC17500.028.4466481
CGCTCTG10550.027.9816475
TCTCGCT10600.027.8730182
GTTACAC6000.027.6219643
GTGTTAC8550.027.0024971
CAATACT6850.026.277944
GGTATAC4450.025.94061
GTATTAT6150.024.6356621
GTGTAAA11450.024.5741881
GTATTAA5650.024.2619231
GTACTAT4303.6379788E-1223.4898171
GTGTATA11450.023.3139741
GTGTAAT8050.023.302231
CTCGCTC12100.023.225083
GTGTTAT9750.022.939141
GGGTTTC33100.022.8866041
Produced by FastQC (version 0.11.5)