FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326918
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9150.035.406498
GTCTTGC18750.034.06331
GTCTCGC10700.033.2366831
GTACCGA1352.1276974E-532.2569121
GTATTAG6750.030.1064531
GTATTCG1504.3689928E-529.0312231
GCCGATC2054.2988358E-728.0949068
GTCGTAA1306.4448634E-427.9146371
TCGCTCT11400.027.786854
GTATAAT6700.027.0813641
GTGTTAG14650.026.752321
GTACTAA3607.2759576E-1226.208741
GTATTAA6700.025.998111
GTCTTAC8700.025.8611451
CGCTCTG12650.025.0405755
GTATTAC4700.024.707421
GCTATCG1751.317708E-424.6833828
GTATAGG5700.024.1926841
GGGTTAA6950.024.0186371
GTTAGCC11750.023.8956173
Produced by FastQC (version 0.11.5)