FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2290717
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.038.1177758
GTCTCGC9300.034.9051131
TCGCTCT9400.032.1703224
TAATACT5200.031.846264
TCTCGCT10850.031.2094822
CTCGCTC10350.029.9301283
GTATAAC4300.028.5193711
GTGTAAC6500.027.745091
GTCTTGC18450.027.369141
GTGTAAA11300.026.8120671
GTACTAT4650.026.3727531
CTGTCGC12100.026.1819089
GTGTAGA11750.025.7852251
GTTTCAC28400.025.1129283
CGCTCTG11200.025.071515
GGGTTTC33100.025.0627841
GTCTCAC23450.024.9173371
CCTATCG1450.001277403724.827679
GGGTACG2051.3423878E-524.6322251
GTATTAA4750.024.2988571
Produced by FastQC (version 0.11.5)