FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378422
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18400.036.8509371
TCTGTCG8050.035.775048
GTCTTAC6800.032.8845061
GTACTAT5050.032.8529931
GTACTAC3450.031.3625011
GTATTAA5600.030.9144651
TCGCTCT9700.028.9473554
TAATACT4350.028.1368644
GTCTCGC9750.028.1136681
CTGTCGC11750.027.573429
GTATTAT5950.026.67131
TACGCTT1358.421387E-426.6656555
ACTCGCG1907.5585267E-626.5253098
TCTCGCT10700.026.261872
TAACACT9550.025.6325344
GTGTAAT8750.025.5559581
TTGCTCT26100.025.1024954
GTGTTAG12700.024.991221
GTGTAAG9750.024.4145011
GTGTAAC6250.024.236941
Produced by FastQC (version 0.11.5)