FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378422
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10450.036.0874941
TCTGTCG8850.034.1661388
GTCTTGC18750.033.65021
TCGCTCT12000.031.7968794
GTATTAT6300.029.9297051
CGCTCTG12000.028.7971735
CTCGCTC13050.028.686843
TCCGTAC1555.7731202E-527.8682333
TCTCGCT13750.027.7780362
TATCCGT1050.0079791827.4258826
TAATACT5950.026.6192384
GTGTACG4100.026.5324331
GTCCTAT5750.026.4862881
GTATTAG6350.026.2678051
ACCGTGT7400.026.2676938
TGTACGG2259.606811E-725.6233442
GTCTTAC8150.024.9155271
GTACTAG4101.8189894E-1224.7636031
GTGTAAC6750.024.7111931
TTACTCT10250.024.5829544
Produced by FastQC (version 0.11.5)