FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630174
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.039.1515358
GTCTCGC10800.037.397521
TCGCTCT11150.034.2237434
GTCTTGC20750.034.063281
CTGTCGC14900.033.8250359
TAATACT4900.032.3260884
TCTCGCT12300.028.1158832
ACCGATC1555.77003E-527.8705868
GTATAAT6850.026.322551
TCTCGAT9850.026.314367
CTCGCTC13250.026.0970483
GTCTCAC26000.026.0755231
TCGATCT10650.025.6897899
GCGTTAC1701.0690513E-425.4554541
GTACTAT5400.025.3768881
CGCTCTG12950.025.018965
GTGTTAC8500.024.606941
GTATTAG6450.024.600361
CTCGATC10850.024.5526588
TACACTG13500.024.5329975
Produced by FastQC (version 0.11.5)