FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438737
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA6600.033.8882181
TCTGTCG7850.028.4335868
GTATTAT6450.027.9647731
TTACACT9150.027.5414354
GTCTCAC19450.026.337211
TATACTT5950.025.4121825
TCGCTCT7400.025.297714
GTGTTAG13050.024.8790041
GTCCTAT6100.024.8382191
GTACTAG4101.8189894E-1224.6362821
TACACTG11400.024.6319835
GGATAAC6800.024.4033761
GTCTCGC8700.024.0497041
TAATACT6000.024.0003934
GTCTTAC8600.023.490411
GTATTAC5050.022.8591251
GTGCTAT8250.022.73791
GTCCTAC5100.022.6350151
GTGTTAC6700.022.6139011
CTATACT5750.022.53954
Produced by FastQC (version 0.11.5)