FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855038
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC22700.036.792621
TCTGTCG10450.036.5170568
TCGCTCT12300.034.5362744
GTCTCGC11050.034.5336271
GTATAAG7450.029.9594631
GTGTAAC7650.029.1762051
CGCTCTG13700.028.9048485
GTTTCAC36050.028.5600763
GGGTTTC39400.027.9591391
TCTCGCT13750.027.7524762
GTGTTAG16700.027.5925671
TACCCGT2654.9167284E-927.1695675
GTCTTAC9400.026.8082681
GTATTAA7000.026.7426171
TTACGCT1959.246027E-625.845924
GTATTAG7550.025.7481121
GTATTAC5950.025.4115371
CTCGCTC15450.025.164823
GTGTAAT10650.025.0138591
GGTATAC5800.024.8273641
Produced by FastQC (version 0.11.5)