FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855038
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10850.041.1655241
TCGCTCT11700.036.3073354
GTCTTGC24050.030.8527451
TCTCGCT14700.030.3670482
TCTGTCG12350.030.3165538
TAGTACT6450.030.1392384
TTCACCG7350.029.3874665
CTCGCTC14000.028.7997173
GTGTTAG17200.028.4807971
CGCTCTG14900.026.5769185
GTTAGCC14950.026.0064333
GTCTCAC27950.025.7744771
GTGTTAC11000.024.886431
GTGCTAC9400.024.5241411
GTACTAC4700.024.5241411
GTATTAT7700.024.325081
ACCGTGT8650.024.1393388
GTATATA10500.024.0132221
TTGCTCT31200.023.9997634
TGTTAGC16250.023.9259172
Produced by FastQC (version 0.11.5)