FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022593
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.053.9155438
TCGCTCT12900.039.0697364
GTCTCGC12650.038.1343571
GTCTTGC26150.036.06881
TCTCGCT15150.030.8910682
CTGTCGC19350.030.1400099
CTCGCTC15150.028.9900783
CGCTCTG15550.028.7073755
GTCTCAC28350.028.1904541
GTTTCAC38900.027.2082043
GGCTAGA16750.026.6507241
GGGTTTC43900.025.4213921
GTATAGC6000.025.1999821
GTATAAC6000.025.1999821
TCTCGAT10600.025.1324717
GTCCTAC7800.024.9230581
GTCTCGA25000.024.191986
ACCGTGT9900.024.000388
TAACACT12350.023.9028134
CTCGATC10750.023.442238
Produced by FastQC (version 0.11.5)